To 2012 a nested RT-PCR assay [21] Prior, which amplified a 143-nt region in the E1 coding region, was performed using GoScript Change Transcriptase and GoTaq Flexi DNA Polymerase (Promega, em /em Madison, WI, US) based on the producers instructions, accompanied by gel electrophoresis

To 2012 a nested RT-PCR assay [21] Prior, which amplified a 143-nt region in the E1 coding region, was performed using GoScript Change Transcriptase and GoTaq Flexi DNA Polymerase (Promega, em /em Madison, WI, US) based on the producers instructions, accompanied by gel electrophoresis. age group, also to strengthen lab and epidemiological investigations of suspected … Continue reading To 2012 a nested RT-PCR assay [21] Prior, which amplified a 143-nt region in the E1 coding region, was performed using GoScript Change Transcriptase and GoTaq Flexi DNA Polymerase (Promega, em /em Madison, WI, US) based on the producers instructions, accompanied by gel electrophoresis